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KMID : 0604520150410040391
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Journal of the Society of Cosmetic Scientists of Korea
2015 Volume.41 No. 4 p.391 ~ p.400
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Antioxidative Effects and Tyrosinase Inhibitory Activities of Mate (Ilex paraguariensis) Extract/Fractions
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Kong Bong-Ju
Kim Yong-Jae
Baek Jee-Seon
Lee Da-Bin
Lee Ji-Won
Min Na-Young
Kim A-Young
Park Soo-Nam
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Abstract
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In the present study, 50% ethanol extract, the ethyl acetate and aglycone fraction were prepared from mate (Ilex paraguariensis) and their antioxidative ability was evaluated. The yields of extract and fractions were 32.0, 4.48 and 0.82% per dried powder, respectively. Free radical scavenging activities were performed by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) assay and total antioxidant capacity was estimated using luminol-dependent chemiluminescence assay. Free radical scavenging activities (FSC50) of 50 % ethanol extract, ethyl acetate fraction and aglycone fraction were 8.83, 5.84 and 6.05 μg/mL, respectively. Their total antioxidant capacities (OSC50) were similar to that of L-ascorbic acid (1.72 μg/mL), known as a prominent water soluble antioxidant, in all extracts and 50% ethanol extract (1.03 μg/mL) was the most effective. The cellular protective effects on the 1O2-induced cellular damage of erythrocytes were evaluated and the results showed that all extracts were significantly higher than (+)-α-tocopherol at 10 μg/mL. Especially, the τ50 value of aglycone fraction was 5 times higher than (+)-α-tocopherol at 10 μg/mL and 50 μg/mL. The inhibitory effects of the ethyl acetate and aglycone fractions on tyrosinase were similar to arbutin, known as the whitening agent in cosmetics. These results suggest that the extracts of mate have the applicability as antioxidant and anti-aging cosmeceutical ingredients.
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KEYWORD
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Ilex paraguariensis, antioxidant activity, DPPH, cellular protective effect
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